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การประชุมวิชาการชีวภัณฑ์ชนิดใหม่ของ Pseudomonas fluorescens SP007s มีความเสถียรในการควบคุมโรคขอบใบทองและส่งเสริมการเจริญเติบโต ของพืชคะน้า (2017)ผู้แต่ง: Dr.Tiyakhon Chatnaparat, Assistant Professor ,  ธาณิกานต์ เนียมแตง, ณัฐกานต์ สาตราภัย, ลาวัลย์ กลัดสุวรรณ, มะลิดา ชูรินทร์, ดุสิต อธินุวัฒน์, Dr.Supot Kasem, Assistant Professor, Dr.Sutruedee Prathuangwong, Professor , การประชุมวิชาการ: 
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Extracellular Proteome of Bacillus amyloliquefaciens KPS46 and Its Effect on Enhanced Growth Promotion and Induced Resistance Against Bacterial Pustule on Soybean Plant) ผู้เขียน:ณัฐธิญา เบือนสันเทียะ, ดุสิต อธินุวัฒน์, Tiyakhon Chatnaparat, Gary Y. Yuen, ดร.สุดฤดี ประเทืองวงศ์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractBacillus amyloliquefaciens strain KPS46 is gram positive that has been found to be effective in controlling various diseases and improving crop yield under greenhouse and field conditions. This study was investigated for KPS46 ability to activate extracellular protein elicitors in enhanced plant growth and induced systemic resistance of soybean plants. Soybean cv. Spencer seeds treated with KPS46 increased root and shoot length, biomass by more than 20-40% and the reduced bacterial pustule disease severity up to 50% when compared with the non-treated control. Bioassay data showed that seedling growth phenotype increased by KPS46 was significant upregulation of 20- elicited extracellular protein spots, whereas sterile distilled water had no effect on either enhanced plant growth or induced resistance. To investigate the proteins involved in growth promotion and induced resistance mechanisms, two-dimensional gel electrophoresis was used to separate extracellular proteins secreted by KPS46 wild type and by N19G1, a UV-derived mutant of KPS46 with reduced production of extracellular proteins and lacking growth promotion and induced resistance activity. Using the 2D-PAGE, the extracellular protein of B. amyloliquefaciens KPS46 grown in nutrient glucose broth was studied. The identity and putative function of twenty proteins secreted by KPS46 but not by N19G1 were determined. The 20 extracellular protein spots were identified that were known to be secreted by various mechanisms. These extracellular proteomes of the strain KPS46 included proteins from different functional classes and some proteins of yet unknown function. To our knowledge, this was the first two-dimensional extracellular proteome map of a PGPRs, KPS46. The analysis revealed a number of proteins which might be involved in plant growth promotion and induced resistance by acting as plant growth regulators, accumulating bio-fertilizer/ nutrients, producing antibiotic compounds, stimulating metabolism or functioning in defense against stress factors. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Pectate lyase from Xanthomonas axonopodis pv. glycines 12-2 and Associated Pili Transporter Constitute a Key Virulence Factor) ผู้เขียน:วิลาวรรณ์ เชื้อบุญ, ดุสิต อธินุวัฒน์, ดร.สุดฤดี ประเทืองวงศ์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractA recent study has demonstrated that XagP in xagP of Xanthomonas axonopodis pv. glycines 12-2 )Xag 12-2), the causal agent of soybean bacterial pustule, is required to induce a hypersensitive response (HR) in tobacco (Nicotiana rustica). Until now, its role in virulence on soybean was unknown and whether the general secretion pathway (GSP) is different from other bacteria. To understand the possible relationship between functions, xagP and T4P operon-corresponding to XagP secretion and type IV pili of Xag 12-2, respectively- were mutated using the site-directed method. XagP secretion into media associated with ?xagP and ?T4Pmutants was reduced by 100 and 24.8% respectively. Pili mutants were less reduced in the Pel secretion than by other GSPs which might be necessary for an XagP transporter. Both mutants elicited HR-like typically necrotic symptoms which were the same as the wildtype, that is, within 48 hr after infiltration on susceptible soybean cv. Spencer. On this same host plant, disease severity induced by ?xagP and ?T4P mutants was highly reduced by 82.8 and 55.9%, respectively. It is also interesting to note that the HR and disease symptoms produced by the mutants were different from the wildtype. The mutants neither expressed haloes surrounding the HR nor scattered lesions over spray-inoculated leaves but revealed pustules around the leaf margin at hydathode pores. Complemented mutants showed enhanced secretion, transportation, HR and disease severity similar to the wildtype. This is the first report of a co-regulation mechanism of xagP and type IV pili secretion pathway in Xag 12-2 with bacterial pustule disease in soybean. Details involved in these interactions are discussed. The minimal translocation of XagP that is less coincident with the pili transporter should be further elucidated to determine if it is more dependent on other GSPs and can suppress secretion output of other virulence factors associated with Xag 12-2 pathogenesis. |
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